ubiquitin conjugation of hepatitis b virus core antigen dna vaccine leads to enhanced cell-mediated immune response in balb/c mice

Ubiquitin Conjugation of Hepatitis B Virus Core Antigen DNA Vaccine Leads to Enhanced Cell-Mediated Immune Response in BALB/c Mice

BACKGROUND Nearly 350 million persons worldwide are chronically infected with hepatitis B virus (HBV). Ubiquitin (Ub) is a highly conserved small regulatory protein, ubiquitous in eukaryotes, that usually serves as a signal for the target protein that is recognised and degraded in proteasomes . The Ub-mediated processing of antigens is rapid and efficient and stimulates cell-mediated immune res...

Enhanced Immune Responses of a Hepatitis C Virus core DNA Vaccine by co-Inoculating Interleukin-12 Expressing Vector in Mice

Background: Hepatitis C (HCV) is a worldwide problem without an effective vaccine with more than 170 million chronically infected people worldwide. DNA vaccines expressing antigenic portions of the virus with adjutants have recently been developed as a novel vaccination technology. Objectives: In the present study, a DNA vaccine expressing HCV core protein was developed with IL12 as a genetic a...

enhanced immune responses of a hepatitis c virus core dna vaccine by co-inoculating interleukin-12 expressing vector in mice

background: hepatitis c (hcv) is a worldwide problem without an effective vaccine with more than 170 million chronically infected people worldwide. dna vaccines expressing antigenic portions of the virus with adjutants have recently been developed as a novel vaccination technology. objectives: in the present study, a dna vaccine expressing hcv core protein was developed with il12 as a genetic a...

can Isolated hepatitis B core antibody in HIV-infected patients response to hepatitis B vaccine help to elucidate the cause?

β-Glucan Oligosaccharide Enhances CD8+ T Cells Immune Response Induced by a DNA Vaccine Encoding Hepatitis B Virus Core Antigen

DNA vaccination can induce specific CD8(+) T cell immune response, but the response level is low in large mammals and human beings. Coadministration of an adjuvant can optimize protective immunity elicited by a DNA vaccine. In this study, we investigated the effect of a synthetic glucohexaose (beta-glu6), an analogue of Lentinan basic unit, on specific CD8(+) T cell response induced by a DNA va...

Expression of Hepatitis B Virus Core Antigen in Native and Fusion Forms in E. coli

DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...